One half to two micrograms of sample RNA and a matched amount of reference RNA was labeled using the Powerscript Labeling kit (Clontech, Mountain View, CA, USA) in combination with Amersham Cy dyes (GE Healthcare, Little Chalfont, Buckinghamshire, UK).
Briefly, the RT-PCR procedure involved isolating messenger RNA (mRNA) from a matched amount of zebrafish cells from the site of fin regeneration at the three time points, and using primers specific to each gene to selectively detect mRNA as an indicator of gene expression levels.
Federal officials say the requirement was impossible to meet because no bank would provide such credit without a matching amount of collateral.
We had brought an exactly matched amount of food as the subjects ate during the sea level experiments and the subjects consumed a similar diet as they did at sea level during this period.
FairPoint, which is based in Charlotte, N.C., has assets of $3.2 billion and debts of a matching amount, according to the Chapter 11 petition.
The employer then kicks in a matching amount, often half of the employee contribution.
The other five candidates have each received the maximum matching amount of $91,333 from the city.
Every light bulb has a matching wattage - the amount of energy it is capable of producing.
Never revise only the amount of topics to match the amount of questions you have to answer.
The best proof of the relevance of such models is that they have attracted co-funding from a variety of stakeholders, matching the amount committed by the Fund itself so far.
