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The score of a given alignment is the sum of correlations between corresponding segments.
By comparing these two rich evolutionary models, PhyloCSF can observe many additional informative features of a given alignment compared with the d N / d S test.
For example, when we estimate the functional score of a given alignment, there is rarely >15 20% of pairs with GO annotations.
The score of a given alignment with folding takes into account both standard alignment elements such as character matchings, substitutions and indels, as well as the folding score.
Each of these methods produces a set of residues that are potentially important for functional differences between subfamilies of a given alignment.
All remaining RNAz hits of this control screen are then shuffled once again (2) using a more stringent shuffling method that explicitly shuffles all columns of a given alignment randomly (cp. Tab. 3).
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Furthermore, it implements a sequential, PThreads-parallelized and MPI-parallelized algorithm for computing all quartets or a subset of quartets for a given alignment.
Once the maximum likelihood for all available models is estimated and merged into a file by PalmDaemon, statistical information of the parameters can be accessed to evaluate the fitness of a model for a given alignment.
The (secondary structure) model itself is based on a SCFG that provides a distribution of secondary structures for a given alignment.
Then one run of the method involves only summing corresponding pre-calculated values for all 20 amino acids, and for a given alignment of ~100 sequences of length ~400 aa it takes approximately 50 ms (AMD Athlon™ 64 Processor 3800+).
However, the group was not sensitive to phylogenetic model selection as both MrBayes and phyML generated consistent topologies for a given alignment irrespective of the substitution model.
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