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They were positioned by placing their right foot in the right upper quadrant and the left foot in the left lower quadrant of a custom built cadre that was removed after positioning.
Additionally, the cell under study was continuously superfused by means of a custom built close delivery system with a pipette of tip diameter 200 μm placed ∼300 μm from the cell.
Retinas were rapidly dissected under infrared illumination into room temperature, oxygenated (95% O2, 5% CO2) Ames medium and placed in a recording chamber on the stage of a custom built electrophysiology set up.
Second, each subject scan was accompanied by a scan of a custom built phantom that was centrally evaluated for signal-to-noise and geometrical fidelity to ensure performance of the MRI scanners remained within a specific tolerance limit.
The MEA dish was continuously perfused with feeding media (described above) at a rate of 2.5 ml/day by means of a custom built perfusion system based on an ultra-slow flow peristaltic pump (Instech Laboratories Inc., Plymouth Meeting, PA, USA) using an imbalanced set of silicone tubes.
The strengths of a custom built PC are focused on price, performance, and freedom.
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A programmable waterbath was used to maintain constant test temperatures of 20, 25 and 30°C on a custom built thermal arena.
The nanofibers in these tubes were circumferentially aligned by applying small amounts of shear in a custom built flow chamber prior to gelation.
As our methodological overview illustrates, magnetic tweezers are an extremely useful tool for the characterization of helicases and a custom built instrument can be specifically tailored to suit the experimenter's needs.
In order to demonstrate the utility of this technique for planetary exploration and astrobiological applications, we interrogated three sets of samples using a custom built Raman instrument equipped with a deep UV (248.6 nm) excitation source.
"This was followed by a period of scraping images with a custom built scraping tool, by Adam Harvey, for Google and Flickr," notes Dullaart.
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CEO of Professional Science Editing for Scientists @ prosciediting.com