Sentence examples for of a crea from inspiring English sources

In fact, introduction of pDCre1000 into YP-4 precluded the generation of a creA disruptant, indicating that pDCre1000 was integrated through non-homologous recombination.

Subsequently, the Febit system was used to investigate carbon catabolite repression by comparing the gene expression of a creA deleted mutant strain with a reference strain grown either with glucose or ethanol as the sole carbon source.

The maximum specific growth rates of A. nidulans wild type strain and that of a CreA deletion strain have been observed to be 0.25 h-1 and 0.11 h-1, respectively, when grown on glucose [ 19].

In order to monitor the dynamics of CCR in A. nidulans a CreA::GFP tagged protein under the control of the native promoter was constructed, enabling the study of CreA nuclear localisation under repression/derepression and the evaluation of the signalling components that led to CreA cellular compartmentalisation.

This approach was applied to analyze expression data concerning a reference strain and a creA deleted strain of A. nidulans, grown on different carbon sources, specifically glucose and ethanol [ 34].

The regulation of CreA-mediated carbon catabolite repression (CCR) in the parental strain was determined by fluorescence microscopy, utilising a CreA::GFP fusion protein.

Although d-glucose generally represses the expression of amylolytic genes in a CreA-dependent manner (Felenbok and Kelly 1996), it has also been shown to induce α-amylase in A. oryzae (Carlsen and Nielsen 2001).

In our previous report, expression of amyR was shown to be repressed in a CreA-dependent manner (Tani et al. 2001b .Expression of amyR was highest on starch followed by maltose, and decreased on d-glucose and glycerol in the creA+ strain of A. nidulans.

We and others have previously shown that loss-of-function of CRE1 or CreA leads to an alteration in nucleosome repositioning upon addition of glucose [ 32- 34].

YDCre generated a 7 kb band, indicating a deletion of the creA gene (Figure 2).

A modulation of CreA derepression was subsequently identified as the mechanism by two central NPKs, schA and snfA, controlled hydrolytic enzyme production.