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The supply of air to an enclosed space involves the removal of a corresponding volume of expired air, which may be laden with odours, heat, noxious gases, or dust resulting from industrial processes.
Injection of a corresponding volume of vehicle solution (150 µl normal saline) also caused a decrease in HR (Figure 2C) and blood pressure (Figure 2D), however to a much smaller extent than adenosine.
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Purified PLUNC was added to LB broth to give a final concentration of 100 µg/mL and a corresponding volume of buffer was added to LB broth for the vehicle control.
When required, MLL cells were plated at 60% confluence, allowed to adhere for 24 hours, then treated overnight with 1.5 μM of AZD6244, or with a corresponding volume of DMSO as a carrier control.
When relevant, recombinant archease (to a final concentration of 0.2 μg/μl) or a corresponding volume of buffer (10 mM Tris HCl pH 8.0, 100 mM NaCl, 1 mM dithiothreitol (DTT), 10% (v/v) glycerol) was added to the reaction.
However, to account for the observed temperature and salinity change this would imply a relatively high out-flowing temperature of more than 16 °C and a corresponding volume flow of less than 0.03 m3 s−1.
The animals were treated daily with Ly2811376 at a dose of 100 μg/g of body weight/day for 29 consecutive days, or a corresponding volume of vehicle solution, a treatment that did not change body weight.
After the cells had adhered and spread on the substrates, 2 µM of Cytochalasin B (Sigma) resuspended in DMSO or a corresponding volume of DMSO was added to separate plates.
The sample (100 μL) and a corresponding volume of 300 μL of buffer were added to the sample and buffer chambers, respectively.
infusion of a 300 maximummaximum 600 mg) cyclophosphamide (treatment arm) or a corresponding volume of saline solution (control arm).
For an AAR0 of 0.6 and an ELA0 rise of 200 m (400 m) we calculate a total area loss of − 54% (− 80%) and a corresponding volume loss of − 50% (− 78%) compared to the 1973 glacier extent.
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