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All genes were required to have a minimum of 2 fold expression and 100RFU for raw signal.
Starving fourth instar nymphs were forced to ingest 50 µL of 2 fold concentrated human sera containing 2.5 µg/ml of propidium iodide.
The temporal expression pattern of 1857 genes found to be significantly differentially expressed (FDR<1%, minimum of 2 fold change) are shown in Figure 1.
To determine differentially expressed genes a fold change cut-off of 2 fold was used.
A p-value cutoff of 0.05 and a change of 2 fold or more were selected for gene analysis.
Firstly, we tried to identify DEGs by applying screening thresholds of 2 fold changes and Benjamini q value <0.001.
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Out of 17 compounds that were detected in both samples, eight showed increase of ≥2 fold after 24 hours.
However with an AF of >2 fold rise in DHF patients as compared to the healthy controls, this may suggest a probable risk association to DENV infections.
The limit of detection for these assays is <10 pg/mL based on detectable signal <span class="lh">of >2 fold above background.
To identify crucial differentially expressed HSP90 clients in each cell line, expression changes of >2 fold after treatment were obtained.
We identified differentially expressed genes between the conditions analyzed using a cut-off criteria of ≥2 fold log increases for up-regulated genes.
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