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The first is Y OD (OD for off-diagonal), formed from Y B by replacing the diagonal elements (representing domestically produced consumer goods that are also sold domestically) with zeroes; consequently, Y OD contains only the exported consumer goods.
We estimate that OD 5 contained 2.3 × 10 cells applied per vial (Table S4).
Next, we define Y OD, the matrix containing the off-diagonal elements of Y B and zeroes down the diagonal.
Using RCA, we initially generated self-assembled composite DNA microsponge structures containing ODN's in the form of long single-stranded oligonucleotide (ssDNA) polymers that each contain several thousands of repeated ODN copies complementary to a selected target sequence for antisense therapy.
The stimulation index (SI) was calculated as the ratio of the average OD value of wells containing antigen-stimulated cells to the average OD value of wells containing only cells with medium.
Briefly, 5 10 mg of N-acetoxy N-trifluoroacetyl-7-fluoro-4-aminobiphenyl or N-acetoxy N-trifluoroacetyl-7-fluorofluorene dissolved in absolute ethanol was added to a sodium citrate buffer (pH 6.0) containing 200 250 ODs of unmodified ODN and placed in a 37 °C shaker overnight.
The per cent inhibition for each concentration of inhibitor was determined from the following formula: % inhibition=(A0-A/A0) × 100, where A is the OD from the well containing the inhibitor (corrected for background) and A0 is the OD from the well containing no inhibitor (corrected for background).
The optical density (OD) of the specimen well was compared with the OD of the well containing a calibration cut-off (CO) serum sample provided with the kit.
The end point titers were determined as the highest dilution of serum for which the OD was 3SD above the mean OD of control wells containing serum from naïve mice.
Selected cultures containing 36 OD were treated with either 500 µM or 1 mM spermine NONOate for 30 min. The cultures were pelleted by centrifugation at 4°C, washed with 50 mM Tris-HCl buffer, pH 7.6 and the cytoplasmic proteins extracted in 400 µL B-PER (Pierce, Rockford IL) after vortexing for 30 sec.
For cultures containing CaCO3, OD was determined by diluting the sample 6-fold with 4 N HCl to solubilize CaCO3.
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