For all assays (training, memory test and olfactory acuity), odorants were diluted in mineral oil at a final concentration of 0.36 mM for octanol and 0.325 mM for methyl-cyclohexanol, and were delivered by 0.4 l/min airflow bubbled through odor-containing bottles.
"For all assays (training, memory test and olfactory acuity), odorants were diluted in mineral oil at a final concentration of 0.36 mM for octanol and 0.325 mM for methyl-cyclohexanol, and were delivered by a 0.4 L/min flow bubbling through odor-containing bottles".
HS flies were also strongly impaired in associative odor learning, while memory decay, sensory acuity and basic motor behavior remained largely unaffected.
Secondly, we wanted to determine if odor fear conditioning, which modifies olfactory acuity and piriform cortex evoked activity, also modifies sleep structure recorded within the piriform cortex itself during the post-conditioning period.
In electric shock-avoidance controls, one arm of the T-maze was electrified with 80 or 120 V dc for 2 min. In odor-avoidance controls, flies were exposed to 5×10−3 or 1×10−2 dilutions of MCH or OCT versus air for 2 min. A performance index represents the average normalized percent avoidance of the shock-paired odor (learning, memory) or individual stimulus (sensory acuity).
This enhanced SWS may contribute to and/or facilitate odor memory consolidation leading to learned changes in perceptual acuity and changes in learned fear behavior to the conditioned odor.
To understand whether individual differences within the normal range of peripheral olfactory acuity were related to anosmia for the asparagus odor, an index of performance on asparagus perception (proportion of correct choices) was correlated with the olfactory threshold for phenyl ethyl alcohol and the resulting r value tested to see if it differed from zero (Edwards 1973).
We show that a daily hyperthermic episode throughout larval and pupal development (1) severely disrupts MB anatomy by reducing intrinsic Kenyon cell (KC) neuron numbers but has little effect on other brain structures or general anatomy, and (2) greatly impairs associative odor learning in adults, despite having little effect on memory or sensory acuity.
Odors and electric shock were applied in the same manner during conditioning as when testing for sensory acuity.
