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This was both to control the mutation rate by ensuring that the reaction did not saturate the reagents, which would cause the number of doublings to become sensitive to the initial template concentration, and to avoid the PCR-based recombination events that can occur during with the last few thermal cycles of PCR reactions [ 38, 39].
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There is conflicting information in the literature with respect to metabolic changes that occur during CPB with respect to the use of fresh or old blood.
Disturbances of this system with resultant bleeding complications are known to occur during therapy with mild hypothermia [ 5, 13, 14, 17].
This scenario is more likely to be associated with excessive alcohol consumption or fat accumulation, or occur during infection with HBV or HCV.
To assess difficulties that occur during treatment with the Ilizarov external fixator, Paley's classification was used [17] to distinguish problems, obstacles and complications.
When 5 emergencies occur during operation with BI of 3000 ms, we can see that every superframe must include 15 EGTSs to satisfy the emergency requirement.
Teriparatide is indicated in severe osteoporosis or when new vertebral or hip fractures occur during treatment with other approved drugs for osteoporosis (grade A recommendation).
In addition, recombination may occur during infection with multiple viruses of laboratory cell-lines.
Anemia can also occur during treatment with cisplatin.
Secondly, elevation in plasma [Na+] is likely to occur during treatment with a higher dialysate [Na+].
In recent years it became apparent that GI perforations also occur during treatment with antiangiogenic tyrosine kinase inhibitors (TKIs).
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