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Our data indicate that any potential redundancy between Cul2 and Cul5 in Drosophila must occur by utilizing different Roc proteins, as Roc1a is not part of a Cul5 complex, and Roc2 is not part of the Cul2 complex.

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In conclusion, we have demonstrated that by utilizing naturally occurring amino acid substitutions the toxicity of the four T cell epitopes in α-gliadins can be eliminated.

We next examined the promoter region of PTEN gene to confirm if epigenetic modification such as DNA hypermethylation occurred in EOL-1 cells by utilizing methylation-specific PCR.

By utilizing naturally occurring surface proteins as scaffolds, functional proteins can be displayed on various cell surfaces (Hansson et al. 2001).

Cell based transgenesis allows investigators to make more precise genetic modification by utilizing natural occurring events within the genome such introduction of double stranded DNA breaks at targeted sites.

The system parameters under investigation are all time-varying and the randomly occurring filter gain variations are modeled by utilizing a random variable obeying prespecified binary distribution which is uncorrelated with other stochastic variables.

The phenomena of the randomly occurring uncertainties and packet dropouts are characterized by utilizing mutually independent random variables with known occurrence probabilities.

The sequence encoding the human CD81 gene (NCBI Accession # BC002978) was optimized for mammalian codon usage by utilizing the codons predicted to occur frequently in highly expressed genes of mammals [21].

By utilizing the morphological changes that occur in both apoptotic and necrotic cells, the samples were stained simultaneously with Annexin V-FITC and propidium iodide.

In this work, we utilized an ISA-based detection strategy, which is a variation of a standard ELISA, in which fluorescence detection can occur by simply having either a primary or secondary antibody coupled to a fluorophore.

Awareness of the pathways utilized by midgut cells to contend with toxin damage may allow us to better predict possible resistance mechanisms that could occur by field application of Bt formulations.

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