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Within a discrete framework, time values are defined as distinct time periods or measurement occasions; in this study, pretreatment, post-treatment, 6-month follow-up and long-term follow-up measurements were categorised into four separate assessment occasions irrespective of the actual duration since baseline.
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Measurement of inflammatory markers was performed on one occasion in this study.
This one final questionnaire is then sent out to the study participants by mail at one occasion (in this study: survivors, between February and June, 2008; controls, between September and November, 2008).
We utilized Vienna RNA package [ 7] to fold RNA (The default parameters are used in the all occasions in the study).
No significant differences in length of MRSP carriage appeared either between the three diagnostic categories (p = 0.31) or in the dogs with dermatitis on the following sample occasions in the study compared to dogs with no visible signs of skin disorder (p = 0.78).
The method is based on a proportional hazards model in which covariates are incorporated parametrically, with the logarithm of the baseline hazard function approximated by a smooth function of the observation interval midpoints; it allows for an arbitrary observation scheme, with subjects seen at irregular intervals and on a varying number of occasions, as occurred in this study.
On many occasions in our study, this role of minimally intrusive observer could be achieved, especially as families and HCPs were reported to be used to observers being present (e.g. trainee and visiting HCPs), with some HCPs commenting that in time they 'forgot' that RN was present.
All dogs received systemic treatment with antimicrobial agents to which the cultured MRSP were resistant prior to the first sample occasion in the study.
The first dog (dog no 1) was treated for three weeks and was found to be negative on the very first sample occasion in the study, 8 months (34 weeks) after the inclusion sample.
Of particular concern in this study were occasions when the researchers encountered people who were being treated within the shrine and prayer camps and presented with severe and distressing symptoms.
Notable in this context is the fact that the horses in this study on many occasions had intact epithelium covering the corneal lesions, as demonstrated by negative Fluorescein dye tests.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com