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However, no candidate gene appeared obvious to sequence in the region.
We mapped hazelnut lethality to overlapping deficiencies in the 28E3-29C1 region; however, no candidate gene related to the genes mentioned previously was obvious to sequence.
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Domains or motifs that mediate Swf1 or Pfa4 specificity are not obvious to identify by sequence comparison, since even orthologues from closely related organisms display low conservation outside the DHHC region, the TTxE motif and the PaCCT motif [24].
The upstream portion of the falcipain-2 prodomain bears no obvious resemblance to sequences of non-plasmodial proteases, and mediates enzyme trafficking to the parasite food vacuole (9).
The advance from animal to human preparations had obvious advantages linked to sequence homology with the native human protein, reducing the incidence of antibody formation that led to injection site reactions and blocking of therapeutic effect.
Whole-genome sequencing can provide structural variation information, but it is not obvious to what extent short read sequences can capture CNV, such as those of only a few hundred base pairs that may delete or duplicate single exons [ 27] or delineate complex rearrangements, given the information filtering step required in matching short reads to a haploid human reference genome.
In the absence of any obvious candidate gene, we decided to sequence the whole genome of four affected cases, which were homozygous for the shared identical-by-descent (IBD) segment, and eight unrelated Belgian Blue controls selected as non-carriers for the disease haplotype.
About three-fourths of the assemblies showing obvious similarity to protein sequences were estimated to contain start codons, indicating the high efficiency of cloning of entire mRNA molecules by the construction of full-length-enriched cDNA libraries (Table 2).
The Δ-coordinates of the starting- and maximum-points of the selected probe-trajectories show considerable variation without obvious correlation to their sequence characteristics.
The term 'novel' is being used here to refer to genes with no obvious similarity to a model-organism sequence: i.e. new to scientists, not necessarily to the charophytes.
The C561 G566/C572 A577 sequences lack obvious complementarity to pre-rRNA sequences and they are dispensable for interaction with 35S pre-rRNA.
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