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CLSM analysis showed that archesporial cells with a big and obvious nucleus were formed in the primordia.
"Other" structures included pTDP-43 immunoreactivity in non-p25α-immunoreactive glial cells with an obvious nucleus, potential p25α immunoreactive pTDP-43 extra-axonal inclusions (not associated with a nucleus), or pTDP-43 inclusions detached from any other identifiable structures, such as dystrophic neurites.
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At 72 and 96 h time-points, there are cells with obvious lobular nuclei or BN (banded nuclei) and GN (cytoplasmic granules), both hallmarks of granulocyte differentiation [ 14, 33]; Figures 3(C) and 3(D).
The amount of Nup88 was altered in all of the three compartments analyzed here; these changes were most obvious in the nucleus and at the NE.
The most obvious difference between nuclei from different fixation procedures is a distortion of nuclear shape by some methods.
Among these nuclei, the aggregate-associated neurodegeneration was most obvious in the paraventricular nucleus (PVN).
In general, apoptotic cells have obvious morphological changes in nucleus and cytoplasm, including bubbling of cell membrane, degradation of cell nuclei with widespread damage of chromatin, and fragments of DNA caused by Ca2+ dependent endonuclease [ 34].
Foscan® presents an intracellular fluorescence distribution in cytoplasmic compartments with no obvious fluorescence in the nucleus.
GP80-V5/His was diffusely expressed with enrichment in the nucleus without obvious p62 colocalization (Fig. 3a, third row).
Moreover, by day 9, cells attained myelocyte morphology with oval-shaped nucleus with obvious differentiation-like morphology even in G-CSF-untreated mock transfected cells in addition to siE6AP and E6AP-C843A-transfected E6AP-C843A-transfected E6AP-C843A-transfected
In this context, it is surprising that 16% of the reconstructed embryos develop to the blastocyst stage when 60% of donor nuclei have obvious DNA damage and raises the possibility that damaged DNA in the donor nucleus might be repaired by factors present in the oocyte.
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