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Evolutionary engineering was used to obtain a stable isolate of S. stipitis NRRLY-7124 sthatn that would tolerate the inhibitory compounds present in HSSL, thereby enabling growth and bioethanol production without preliminary detoxification step.
Stable isolated particles and their clusters were obtained by dropping sulfonated polystyrene/THF solution into poly(4-vinyl pyridine)/methanol solution.
A stable isolate, C4, with an improved performance in undetoxified HSSL compared to the parental strain was successfully obtained from it.
Next we determined whether we could obtain stable cell lines from STAT5bERpos memory B cells isolated from clonal density cultures.
The cells were subjected to selection with puromycin (3 ng/ml) to obtain stable cell lines.
Slideshow: EasySense uses a circular antenna array to obtain stable measurements irrespective of orientation.
EasySense uses a circular antenna array to obtain stable measurements irrespective of orientation.
We obtained stable results.
In contrast, slices are usually less rapidly isolated in aged rats and electrophysiological recordings are often delayed in these animals due to difficulties in obtaining stable fEPSPs.
We obtained stable transformants using transposon-based gene transfer technology.
Human K562 cells [ 47] were used to obtain stable transfectants.
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