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We outline a SEQRS experiment focused on obtaining the specificity of a multi-protein complex between Drosophila RBPs Pumilio (Pum) and Nanos (Nos).
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We never obtained the specificity we sought, but Leo did make several monoclonal antibodies reactive to determinants expressed by terminally differentiated cytotoxic T lymphocytes that were clearly not reactive to the desired T cell antigen receptor.
In each case, we obtained the specificity (Si) and Target Specificity (TSij) for the genes studied in the datasets, which allowed for the selection of putative overexpressed genes in cancer or normal tissues as well as the discrimination of genes preferentially expressed in a given condition.
Conversely, for obtaining the highest specificity and PPV, the highest cutoff was obtained at quartile 0.75.
There are a variety of methods for obtaining the sequence specificity of TFs.
At the cutoff values recommended by the manufacturer, the specificity was lower than that of anti-CCP, as previously reported [ 20, 25], and we adjusted the cutoff to obtain the same specificity as that of anti-CCP.
Subsequently, under selection pressure, the enzyme would lose its second acyl pocket Phe residue to obtain the substrate specificity of BChE in the higher vertebrates.
Moreover, the enhanced L1/2 net obtained the best specificity in Model 2 (98.7%) amongst the other approaches.
We adjusted the cutoff values to obtain the same specificity as that of anti-CCP antibodies (94.2%).
Thus, the complementary probe sequence could be chosen from any position within the nucleic acid target to obtain the highest specificity and capture efficiency.
We adjusted the cutoff value of anti-MCV (28 U/mL) to obtain the same specificity as that of anti-CCP antibodies.
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