However, using this length of spacer, we were unable to obtain expression in the pQE vector, tagged with a 6× His sequence at the NH2-terminus, stressing the importance of the 5′-coding sequence in the expression efficiency.
To determine the significance of the obtained expression in each condition, a robust estimation of the noise in the expression data was carried out for each experiment (red curve, see Additional file 1, Figure S1).
Assuming a Weibull statistical distribution of defects, for the CDR configuration with no overpressure, we obtain expressions in closed form for the effective area, a parameter that allows the re-scaling of the experimental data to a reference condition (equibiaxial stress on a unitary area) according to a criterion of equal failure probability.
We obtained expression profiles in terms of relative expression rather than absolute expression levels by dividing the average expression levels of each gene.
Pre-processing of microarray data : The data was quantile normalized and summarized using GCRMA [ 32] to obtain expression scores in the log2 scale.
Thus, we were able to obtain expression data on APC3 in various breast carcinoma tissues, in conjunction with APC7 expression.
A morphologic control was necessary in order to make sure we obtained the expression in-vivo at the desired stereotaxic coordinates.
We were unsuccessful in obtaining LipA expression in A. niger (data not shown), but obtained high expression in N. benthamiana in transient co-expression of the lipA gene with the suppressor of posttranscriptional gene silencing p19 [ 12].
However, it has been difficult to obtain reproducible expression in the same subset of neurons for direct comparison of neurons expressing different functional proteins.
Note that the curves in Figure 10(b) are not identical because to obtain the expression in (12), we have considered several approximations:,,.
