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Simultaneously, the fluorescence from negative control samples were obtained, stored and analyzed.
Reverse transcription and the cDNA obtained stored at −20°C for PCR under the appropriate conditions.
Both serum and plasma were obtained, stored at −80°C, and used to measure markers of acute phase response and vascular inflammation (Schneider et al. 2010).
Patient characteristics are listed in Table 1.> All tissue samples were obtained, stored and assessed under the same conditions as approved by the Institutional Review Board (institutional review board of the TU Dresden).
The medium was replaced with 1.0 ml serum-free DMEM medium, and the cells were then incubated for 12 h, after which the CM were obtained, stored at -20°C, and used for the following experiments.
Amplicons were analyzed using DGGE, excised until a single band was obtained, stored at − 20 °C, and verified according to their reference site in DGGE gels by comparing gels using BioNumerics software.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com