To profile pNP expression, we mapped the obtained stage-specific Illumina paired-end reads to all pNP transcripts.
Fertilized White Leghorn chicken eggs (E. Pavillard, Orny, Switzerland) were incubated at 38°C to obtain stage HH 3 4, stage HH 8, or stage HH 11 embryos [27].
Adults were bred in spawning cages according to established protocols to obtain staged embryos (Westerfield 2000).
It offers the possibility to obtain staging systems for other diseases, as we show here for Alzheimer's disease.
Fertilized chicken eggs obtained from a local breeder were incubated at 37 °C and 80%% relative humidity for the required time to obtain stages 17 18HH.
Embryos were collected and aged at 25° or 18° to obtain stages of interest, and standard protocols for fixing and staining were used.
Fertile Brown Bovan Gold hens' eggs (Henry Stewart, UK) were incubated for 1 16 hr to obtain stages X XIII (Eyal-Giladi and Kochav, 1976) and stage 4 (Hamburger and Hamilton, 1951) (HH).
In future work, it will be important to test this possibility and investigate the expression even closer to obtain stage-specific promoters which are powerful tools to meet different requirements.
Results: Three stages were obtained: acute stage: 2 weeks postinjury; recovery stage: 2 weeks to 6 months postinjury; and chronic stage: more than 6 months postinjury.
Early-stage samples were taken from patients with stage I, IA, and IB cancers, whereas late-stage data were obtained from stage III and IV patients.
