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Furthermore, obtained sequences were processed using the Classifier tool (Wang et al. 2007) of the RDP pyrosequencing pipeline http://pyro.cme.msu.edu/.msu.edu/
The obtained sequences were processed and classified by using modules implemented in the Mothur V.1.20.0 software platform (19 – 22 ).
After clipping of the BAC specific barcodes the obtained sequences were processed following the assembly pipeline depicted in Fig. 2. In the preprocessing phase, reads derived from BAC vector and E. coli DNA were discarded (mean 3% and 8% of all reads, respectively) leaving 1,086,323 sequences with average read length of 221 bp (Table 1) for assembly.
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The amplicons were sequenced and the obtained sequences were aligned with reference sequences from the GenBank.
The obtained sequences were determined.
Sequences were processed by CASAVA (Consensus Assessment of Sequence And VAriation), a propriety bioinformatics pipeline of Illumina, to obtain variant sets.
Only RefSeq sequences were processed.
Overall, 306,958,418 sequences were processed.
The raw Sanger data obtained from sequencing were processed using the Phred-Phrap-Consed package [75].
The raw reads obtained from Solexa sequencing were processed to obtain clean reads by summarizing data production, evaluating sequencing quality, calculating the length distribution of small RNA reads, removing low quality reads and adaptor sequences as described in previous paper [ 41].
Raw data obtained from Illumina sequencing were processed and filtered using Illumina pipeline (http://www.Illumina.com) to generate FastQ files.
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