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The obtained sections were observed under a Morgagni-268 electron microscope under standard operating conditions.
It can be seen that the mantle parts of the obtained sections generally show similar patterns to those in the "central cross sections" (shown in Fig. 3).
Five tomography models were tested, but the obtained sections show common features: paths sampling the CMB region beneath Africa and paths sampling the root of East Antarctica are anomalous.
The obtained sections were analyzed under fluorescent (Olympus IX-70) and confocal microscopies (LSM 510 Meta Zeiss, Oberkochen, Germany).
As controls, we also obtained sections of non-tumor tissue from the region surrounding TNBC and LUM-B tumors.
The obtained sections were plunged within a few seconds into liquid nitrogen until all sections were collected.
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Section 2 contains the main result obtained, Section 3 contains the corresponding proof.
Profile 1 shows the obtained section, and section 2 shows the junction profile (no gap is observed).
Nonetheless, the images and videos obtained (Section 4) do confirmatively show that cones have been resolved.
Following euthanasia, the mandibles and tibiae were resected, fixed, decalcified and processed histologically to obtain sections for H&E and tartrate-resistant acid phosphatase (TRAP) staining.
Macrodissection of FFPE sections to be analyzed by the HER1 VeraTag™ assay was performed to obtain sections with >70% tumor.
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