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"Critically reviewing articles," "understanding the 'methods' sections in papers," "relating methods used to data obtained," "reading about science," "ability to explain concepts," "understanding experimental design," and "critical analysis" were among the top choices in multiple implementations.
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The obtained read coverage was 96.8-fold.
For this, various Perl scripts were developed to determine the overall and individual GC content of the obtained reads.
Results showed that GC content and obtained read length clearly correlate for both GS FLX and the Titanium platform.
The obtained reads were subjected to quality control as follows.
First, adapter and MIDs are trimmed from the obtained reads.
The obtained reads were mapped to a public reference genome.
Of 50,708 obtained reads, 31,860 passed the quality control.
The obtained reads were assembled prior to mapping to obtain longer sequence reads (contigs), which facilitated mapping and annotation.
We obtained reads from both the 5' and 3' arm for 73.4% of the pre-miRNAs.
Subsequently, the obtained reads were aligned onto the contigs sequences and aligned paired ends.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com