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We obtained punched skin biopsy samples from each case-patient; a pinch of biopsy samples were dab smeared on glass slides for examination for.
Briefly, tissue cylinders of formalin-fixed, paraffin-embedded ERC tissue blocks with a diameter of 0.6 mm were obtained, punched from morphologically representative areas of each donor block, and brought into one recipient paraffin block (30 × 25 mm), using a semiautomated tissue arrayer.
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Attempts were made to obtain punch biopsies of soft tissue melanoma lesions from all patients on day 1 (pre-treatment) and day 28 of the first cycle.
Scaffolds were obtained by punching 8 mm-cylinders into ethanol 70% solution for 10 min and then freeze-drying.
We obtained skin punch biopsies from six MS patients at the Yale MS Clinic that were homozygous for the rs7665090 risk variant or protective variant (Supplementary Fig. 5) and generated explant cultures for the derivation of primary human fibroblasts as described25.
Results using the new autosampling technique were of equal quality to those obtained by punching a 3-mm diameter disk from a DBS or DPS, then extracting and analyzing the target analytes from conventional 96-well microtiter plates, with far reduced time per sample.
Bowers does not consider the reverse design an artistic work, but one which is purely mechanical, obtained by punching characters and devices into a steel hub.
Tumor TMA blocks were obtained by punching 2-nm tissue cores from each donor paraffin block.
Most people simply correct their error and accept the answer obtained after punching the numbers into a calculator.
Tumour TA blocks were obtained by punching a tissue cylinder (core) with a diameter of 1.5 mm through a histological representative area of each 'donor' tumour block, which was then inserted into an empty 'recipient' TA paraffin block using a manual tissue arrayer (Beecher Instruments, Sun Prairie, WI, USA) as described elsewhere (Parker et al, 2002).
Tumor tissue array blocks were obtained by punching a tissue cylinder (core) with a diameter of 1.5 mm through a histologically representative area of each 'donor' tumor block, which was then inserted into an empty 'recipient' tissue array paraffin block using a manual tissue arrayer (Beecker Instruments, Sun Praerie, Winconsin, USA) as described elsewhere [ 27].
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