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The obtained preparations were >95% pure and viable.
The proven immunoreactivity of the obtained preparations has become a premise for a further study on their utility in routine diagnosis of human and animal toxoplasmosis as well as in the immunoprevention of T. gondii infection (as the main or supplementary component of the vaccine).
In fact, in our laboratory, where LF production is a routine and frequently performed procedure, we have on rare occasions obtained preparations of LF that produce much higher TTD in the uniquely sensitive Fischer rat model when compared to previously validated standard LF preparations.
Thus, we obtained preparations of amastigotes associated with host phagolysosomal components (LIMP1 positive), mimicking parasite-containing host cell extrusions, which display or are devoid of LAMP1/LAMP2 proteins (after amastigote isolation from MEF-WT and MEF-LAMP1/2KO respectively).
Approximately one order of magnitude difference between results obtained in basic conditions of washing (100 to 400 EU/ml) and prolonged washing (10 to 40 EU/ml) indicates the strict relation between washing conditions or intensity and the level of purity of obtained preparations (Table 1).
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Thus, obtained preparation was used for further analysis.
The obtained preparation was stained by the Papanicolaou method for cytological examination.
The obtained preparation was ultradiafiltered against PBS, using YM10 membrane (Amicon Division, W.R. Grace, Beverly, MA).
The antioxidant and antibacterial properties of such obtained preparation were analysed, in a purpose, to find the best source to generate antimicrobial preparation based on flax.
These values were close to those recorded in the previous report [ 4] when lettuce bearing the S antigen was lyophilised without any protective excipients and the obtained preparation showed good stability over one-year storage at room temperature.
The presence of phospholipids in the buffers was an absolute requirement for obtaining preparations of enzyme that were capable of ATP synthesis after reconstitution into phospholipid vesicles.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com