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The H-PGDS was purified to homogeneity and a single band was obtained on SDS-PAGE analyses.

For example, using salicylic acid (ligand-ID: SAL) for searching, 16 non-redundant protein targets binding with it were obtained on the results page (Figure 3B).

CORRECTION: I mixed up the columns of "signatures needed" and signatures obtained on the petition page in my original piece on this matter.

Primers for cyclophylin and insulin were designed using the Primer Express Software (Applera Europe, Rotkreuz, Switzerland) and sequences can be obtained on the Web page http://phym.unige.ch/groupes/gauthier/index.php.ch/groupes/gauthier/index.php

We have provided a 'Run WWWBlast' option, with which visualization of the resulting alignments would be obtained on the results page.

The criteria for positive protein identification included a score above the MASCOT threshold of chance expectation value (p<0.05), a molecular weight corresponding to the band obtained on the SDS-PAGE, and a confirmation of the highest score protein by MS/MS analysis of two selected peaks.

Further information about the functional profile can be obtained on two additional results pages.

A zymogram of the xylanolytic activity of crude extracts from XynA-, Xyn10A- and Xyn11B-expressing plants (equivalent of 2.5 mg of extracted leaf tissue) was obtained on 12% SDS-PAGE gel containing 0.1% (w/v) birchwood xylan (Sigma, USA), followed by 3×30 min washes in 100 ml of EB to eliminate SDS and renature the resolved proteins.

Figure  6A shows the reducing and non-reducing SDS-PAGE data obtained on days 0 and 28 of the study.

All visitors to the Tower may obtain, on request, a free 64-page heavy-paper brochure telling exactly where the edifice belongs in the hierarchy of mankind's creative efforts.

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