Sentence examples for obtained nucleus from inspiring English sources

The obtained nucleus pulposus tissue was digested in a mixture of 0.01% trypsin and allowed to digest at 37°C for 15 minutes.

The obtained nuclei were then washed and resuspended in methanol: acetic acid in a 3 to 1 proportion.

Newly formed copper nuclei on the substrate were observed using scanning electron microscopy (SEM) and then manually counted to obtain nucleus density data.

Cells were washed twice with PBS, the cell pellet was suspended in lysis buffer (250 mM sucrose, 50 mM Tris-HCl, pH 7.5, 25 mM KCl, 5 mM MgCl2, 1% protease inhibitor (Sigma), 50 mM NaHSO3, 45 mM sodium butyrate, 10 mM β-mercaptoethanol, 0.2% Triton X-100) and centrifuged at 800× g for 15 min to obtain nuclei.

We applied the standard patch-clamp technique to obtain nucleus-attached patches between 20 and 50 GΩ in resistance.

Four subcellular protein fractions were thus obtained, cytosol, nucleus, membrane/particulate and cytoskeletal fractions.

With some improvement, such as extracting nuclei by water bath heating for dewaxing, we obtained intact nuclei with few foreign materials.

Notably, very similar diffusion coefficients and population distributions were obtained from nuclei of HEK293, HeLa, T98G and TP366 cell lines (Figure 5I and S7).

In G. aurea, the efficiencies were calculated only for the CTAB DNA preparation due to limited plant material and low levels of DNA obtained from nuclei.

The obtained free nuclei in suspension were stained for 30' with 6-diamidino-2-phenylindole (DAPI) solution (10 μM DAPI in 800 mM disodiumhydrogenphosphate) [Sigma D9542, Sigma-Aldrich, St . Louis MO, USA] and evaluated for DNA content by flow cytometry (≥2 × 104 nuclei per histogram).

To characterize the physiological properties of neurons in nucleus basalis we obtained recordings from nucleus basalis in acute coronal slices from adult mice.