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We also compared data from hES and hSperm cells to data from previously obtained mouse ES cell surface proteins.
We obtained mouse genes from the Mouse Genome Database, v5.18 (Blake et al. 2014).
We also obtained mouse melanoma B16F10 cells with a silenced HSF1 expression.
We obtained mouse eggs by the ovary puncture and removed zona pellucidae with the acid Tyrode solution (pH 2).
Finally, to conclusively link Mnk1/2 with Cx43 translation and to exclude potential nonspecific effects of the kinase inhibitor CGP 57380, we obtained mouse embryonic fibroblasts from Mnk1/2 knockout mice [ 23].
To corroborate the role of SRC-3 in IgE-dependent mast cell activation, we obtained mouse BMMCs from SRC-3-/ and wild-type mice and treated them with IgE/DNP-HSA.
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After germ line transmission was obtained, mice were maintained by breeding with C57BL/6.
We also obtained mice that express a BAC transgene containing a 121Q AR and its endogenous regulatory elements (BAC fxAR121).
When a stable baseline was obtained, mice were presented with the HFS protocol (see Materials and Methods section).
To obtain mouse embryonic fibroblasts (MEFs), mice heterozygous for the OREBP alleles (OREBP+/−) on a SVJ129/C57BL/6N background were crossbred [38].
DJA performed breeding and assisted with obtaining mouse cell lines, interpreting data, and writing the manuscript.
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