Following baseline subtractions for each genotype, average GAL4 or UAS values were then subtracted from average experimental group values to obtain minutes of sleep gained or lost vs controls.
Pagers, originally developed for use by the emergency services, were adapted to carry the latest news of rare birds, allowing twitchers to obtain minute-by-minute reports of a particular bird's presence – or absence.
A Sidepak™ Aerosol Monitor (TSI, Inc., St . Paul MN) was used to obtain minute-to-minute average, personal breathing zone PM2.5 concentrations.
EMGi values were obtained 30 minutes, 5 minutes, 1 minute and 10 seconds before the micturition episode, at the time of micturition, and 10 seconds, 1 minute, 5 minutes and 30 minutes after the end of micturition.
Plasma pro-BNP concentrations were obtained 15 minutes after the induction of anesthesia (t0), 15 minutes after myocardial reperfusion (t1), and 24 hours after surgery (t2).
A second set of measurements was obtained 3 minutes after leg elevation.
Heart rate and blood pressure were monitored continuously throughout the procedure from which we computed the measures obtained 40 minutes prior to the video presentation (Pre) and those obtained 40 minutes after initiation of the video (Post).
Blood pressure was recorded as the mean of two measurements obtained 5 minutes apart after subjects had rested in a supine position for 10 minutes.
Static acquisitions were obtained 45 minutes after injection of the radiotracer and lasted for a period of 15 minutes (n = 6).
