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We found that males in good condition were more successful in obtaining matings and obtained longer copulations than males in poor condition.
Whereas in 2008, just after the protocol implementation, they showed a reduction in the median total sedation days from 7 to 5 days, in 2013, 5 years later, they obtained longer duration of total sedation days, mechanical ventilation, and PICU length of stay.
Through 5'-3' RACE PCR, we next obtained longer cDNAs containing fully open reading frames.
The N50 size is two times higher when using the 20 kb library, suggesting that we obtained longer MinION® reads when sheared size is increased.
The biopsies were then divided into two groups: biopsies obtained within 18 months from Hyalograft® C Autograft implantation, and biopsies obtained longer than 18 months after surgery.
Thirdly, more patients obtained longer relief periods with more than 30 minutes by the pressure (data not shown in this preliminary report), unlike the analgesia by DNIC which is known to be extremely short-lasting, ceasing within a few minutes.
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Our study corroborated on a larger scale the results of the Hedén single-center study because it was performed across multiple European sites and also obtained longer-term data after implantation.
In the model maltotriose, one of the main products of β-amylase-mediated hydrolysis, is utilized by the D-enzyme and, together with the obtained longer-chain glucans (20LG and 40LG), may participate in starch synthesis.
The main objective is to obtain longer stability period.
All the paired reads were merged by FLASH (Magoc and Salzberg 2011) to obtain longer reads.
In this study, to obtain longer unknown sequences, amplifying long enough single stranded DNA by linear amplification is necessary.
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