Exact(3)
The obtained library diversity explores a comparable sequence space as the donor-derived natural repertoire and, at the same time, is able to access novel recombined diversity due to lack of segmental linkage.
The obtained library consisted of 3.3×106 clones, of which 14 randomly picked clones all contained the scFv insert as assessed by colony PCR analysis.
Based on overlap between pair-end reads, the obtained library sizes were smaller than the expected 150-300 bp and around 50% of the reads contained detectable traces of adaptor sequence, which means that the fragment size was smaller than the read length of 76 bp.
Similar(57)
The obtained libraries were subjected to paired-end sequencing on an Illumina MiSeq desktop sequencer (Illumina, San Diego, CA, USA).
MJD conceived of the project, obtained libraries and generated the backcross panel.
Where the standard libraries had very small inserts, following gDNA normalisation we obtained libraries with the normal size distribution.
The good quality of the obtained libraries could be confirmed based on the previously reported renal transcriptomes (table 1) [ 21- 27].
In order to characterize the phylogenetic composition and gene content of the obtained libraries, inserts from 1,536 randomly selected fosmids from each library were sequenced at both ends.
The obtained libraries were adjusted and pooled at a concentration of 20 nmol and sequencing was performed with 100-bp, paired-end reads on the HiSeq 2000 (Illumina).
Using the Serial Analysis of Gene Expression method, we obtained libraries of short representative cDNA sequences (tags) from the brains of Baikal whitefish and omul.
These were the following library combinations: union of all genes in all normalized and non-normalized leaf libraries, union of all genes in libraries E and L and union of all genes from all obtained libraries.
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