Exact(11)
The obtained libraries were subjected to quality control on Agilent Bioanalyzer (Agilent Technologies #G2943CA) before sequencing them with Illumina HiSeq2000.
The obtained libraries were subjected to paired-end sequencing on an Illumina MiSeq desktop sequencer (Illumina, San Diego, CA, USA).
MJD conceived of the project, obtained libraries and generated the backcross panel.
Where the standard libraries had very small inserts, following gDNA normalisation we obtained libraries with the normal size distribution.
The obtained libraries were adjusted and pooled at a concentration of 20 nmol and sequencing was performed with 100-bp, paired-end reads on the HiSeq 2000 (Illumina).
Using the Serial Analysis of Gene Expression method, we obtained libraries of short representative cDNA sequences (tags) from the brains of Baikal whitefish and omul.
Similar(49)
The obtained library consisted of 3.3×106 clones, of which 14 randomly picked clones all contained the scFv insert as assessed by colony PCR analysis.
The novel methodology presented here demonstrates an efficient strategy for quickly obtaining libraries of diverse 13N labelled peptidomimetics.
However, obtaining libraries enriched in long cDNAs remains challenging for the production of functional proteins.
User-defined costs can include both fixed (e.g., cost of obtaining libraries) and per unit sequencing costs, with default costs the same as used in our study.
Ask at the desk for help obtaining library materials on high shelves.
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