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In our initial studies, using DOTA-Bz-NCS, less than 60% labelling was obtained after 20 min at 45°C, while the thus obtained labelled product was unstable.
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The obtained labeled cell extract contained the whole yeast metabolome.
The obtained labeled RBC suspension was cooled and preserved at 0 °C until injection.
In the course of various preparations, we reproducibly obtained labeling efficiencies ranging from 60 to 80%.
Cot-1 probe was obtained labeling 100 ng of Cot-1 DNA (1 mg/ml, Invitrogen) by Prime-It Fluor, Fluorescence labeling Kit with FITC-dUTP.
From the 5th-round DNA library of the IVV screening in the presence and absence of a bait Jun protein, called bait and bait screening, respectively [21], we obtained labeled RNAs and hybridized them onto the tiling array.
The two spectra obtained, labeled "A" and "B", are shown by the dashed lines for each blend.
However, it is not straightforward to obtain labelled datasets to perform a supervised NTL recognition task.
We compared the same RNA sample at different dilutions, using the "low starting material" amplification procedures to obtain labelled cRNA for the low concentrations (see Material and methods).
Briefly, 750 ng of total RNA were used to obtain labelled cRNA for each sample to hybridize the arrays following Agilent's protocol.
We used 500 ng of total RNA to obtain labelled, amplified cRNA for each sample to hybridize the Illumina Ref-8 BeadChips according to the manufacturer's instructions (Illumina, Inc, San Diego, CA).
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