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Repeatable and concordant genotypes could be obtained in triplicate with one exception at rs7520386.
During each visit, subjects rested supine for 10 min before automated BP and HR measurement (Omron 780 monitor; Omron Inc, Kyoto, Japan) that was obtained in triplicate with a 1-min lapse between measures.
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To avoid measurement error, each measurement is obtained in triplicates with the average of the two closest as the primary value used for data collection.
In order to determine PCR efficiency, standard curves were obtained in triplicate for the qRT PCR primer set with 4-fold dilutions of a reference batch of cDNA (Pfaffl 2001).
Measurements and Main Results: Thermodilution CO (TCO) was obtained in triplicate.
Data were obtained in triplicate for all dilutions.
This ratio was obtained in triplicate in three independent syntheses and conjugations.
Data were obtained in triplicate and expressed as mean and tested for significance by P < 0.05.
Data were obtained in triplicate, averaged, and expressed as mean ± standard deviation (SD).
All values were obtained in triplicate (n = 3).
Zrs and PV curve data were obtained in triplicate for statistical evaluation.
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