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Fifteen and 23 transformants were obtained from transformation with the constructs.
Ura+ colonies obtained from transformation with pRS416 were also analyzed for marker loss and only 2/1185 tested showed LOH.
Spontaneous suppressor mutations in vpsΔ strains were obtained from transformation plates following the procedure described in Calcagno-Pizarelli et al. (2011), and sltA and sltB mutations were identified by sequencing.
Since we were interested only in the presence/absence of the transgenic proteins, we did not use formal replicates, but extracted proteins from each of at least four different positive lines obtained from transformation with each of the three transgenes, and compared to as many biological replicates of the null segregant.
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In other words, the original T-F representation obtained from transformations like Short-Time Fourier Transform (STFT) can be augmented to a third dimension that represents modulation frequency.
To verify HR as the cause of GFP signal loss, individual colonies were obtained from transformations for further analysis.
Such a fragment was obtained from transformants produced by co-transformation with the plasmids pPsaD-GLuc (7 positive transformants), pHRLucP (16 positive transformants) and pHsp70A-GLuc (10 positive transformants).
The structural MRI was normalized with the MNI template using statistical parametric mapping 8 (SPM8; The Welcome Trust Centre for Neuroimaging, London, UK; http://www.fil.ion.ucl.ac.uk/spm) implemented in MATLAB 2010b (The MathWorks, Natic, MA), and 12 parameters were obtained from linear transformation (i.e., affine transformation).
Fischer Tropsch reaction using a mixture similar to that obtained from biomass transformation (biosyngas) has been studied on promoted Co/SiO2 catalysts.
We report a novel "one-pot", convenient and efficient method based on radiolysis to synthesize gold-based nanoparticles finely dispersed on reduced graphene oxide (rGO) nanosheets obtained from reductive transformation of graphene oxide (GO).
The model is obtained from the transformation between the domains of frequency and space using a Rational Fraction Polynomials (RFP) method, which is modified with multiobjective genetic algorithms in order to improve the fitting of track receptance and to assist integration during simulations.
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