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In the presence of TDZ at 0.3, 1 and 3 mg l−1, the somatic globular embryos were obtained from the leaf explants (Table 1).
Nonetheless, a greater proportion of plastome-derived reads, as expected, was obtained from the leaf tissue-derived template, presumably due to greater abundance of chloroplasts within leaf cells.
Senescence associated WRKY genes from other species were obtained from the Leaf Senescence Database (http://www.eplantsenescence.org/) by performing a text search with the term "WRKY".
Extracts obtained from the leaf and stem of Quassia amara and Q. undulate were screened against P. berghei berghei in mice at doses of 100 and 200 mg/kg.
Full length cDNAs of WsCYP98A and WsCYP76A genes were obtained from the leaf tissue of WS-3 rich chemo-variant by degenerate PCR and RACE methods (WsCYP98A: HM585369 and WsCYP76A: KC008573).
This sorbent was prepared by mixing biochar (obtained from eucalyptus leaf residue) with FeCl3 and FeSO4 solutions and the addition of NaOH up to the pH value 10 11.
Information obtained from growth parameters, leaf area and specific leaf area in particular, could be related to essential oil production in leaves of thyme plants.
Total gDNA was obtained from young leaf material through standard cetyltrimethylammonium bromide (CTAB) extraction (Doyle and Doyle, 1987).
Explants were obtained from coffee leaf fragments cultivated in C medium [ 42] modified with 20 μM 2,4-D (C20 medium).
Aluminium chloride obtained from alder leaf, known for its phenolic complexing activity, is also reported to have the larvicidal activity against S. aegypti [ 23].
Porous polytetrafluoroethylene (PTFE) is used as a substrate for imprinting the leaves, resulting in increased signal intensities compared with those obtained from direct leaf tissue analysis.
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