Sentence examples for obtained from testes from inspiring English sources
In non-obstructive azoospermic patients (NOA) besides the mechanical treatment, vital spermatozoa from the tissue obtained from testes by biopsy can be enzymatically prepared.
Male metaphase I and II chromosomes were obtained from testes of the 5th instar larvae.
Spermatogonial germ cell suspensions, including undifferentiated Kit-negative and differentiating Kit-positive spermatogonia, were obtained from testes of immature 7 day post-partum (dpp) mice.
Enriched spermatogonia fraction was obtained from testes of immature 4 or 7 day-old Swiss CD-1 mice, as reported previously [10].
Undifferentiated Kit-negative spermatogonia obtained from testes of 4dpp mice, were purified by depletion of Kit-positive cells with immunomagnetic beads and their purity was monitored by FACS analysis (Fig. 3A).
To establish specific expression at different stages of spermatogenesis, total RNA obtained from testes of prepubertal and adult male mice (age range 8, 10, 12, 14, 16, 20, 30 and 84 days) was used for reverse transcription.
In fact, when Kit-negative spermatogonia (obtained from 4dpp testes, after removal of Kit positive cells) were cultured for 24 h in RCCS, they underwent differentiation, as evaluated by increased expression of genes important for commitment of mitotic germ cells to the meiotic programm (Kit and Stra8), even though they failed to organize Scp3 onto chromosomes.
Furthermore, these isolation methods had a very low efficiency; about one million LHR-negative and PDGFR-α-positive cells were obtained from the testes of 60 pups (with an average of 8 500 cells per testis).
Morphologically, the SSCs from the two ages of mice were indistinguishable, although fewer SSCs were obtained from the older testes than from the younger testes (data not shown).
In the current study, donor spermatogonial cells were obtained from adult tilapia testes.
In 2003, Kanatsu-Shinohara and his colleagues succeeded in culturing SSCs obtained from postnatal mouse testes.
