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Notably, conditioned medium obtained from differentiated EC, and rich in PDGF-BB, was not able to induce expression of PDGFRβ in EC.
At the same time, conditioned medium obtained from differentiated endothelial cells (HUVEC-CM) did not change the expression levels of PDGFRβ.
Comparison of dose response curves obtained from differentiated and stem-like cells showed a highly significant statistical difference (p = 0.019, ANOVA).
Presently, most of the drug for clinical use is produced by semi-synthesis [ 8], starting from a natural precursor, 10-deacetylbaccatin III, which can be obtained from differentiated yew tissues, mainly leaves, with a relatively good yield [ 9, 10].
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Only 5 samples (11%, mean IRS: 0.47) obtained from moderately differentiated HCCs expressed p16 in the nucleus.
Another advantage of using this new approach is that in vitro cultures initiated from seeds minimize the probability of contamination as compared to those obtained from other differentiated tissues, such as leaves and stems.
Furthermore, to be able to distinguish between CF and CFTR miRNAs networks and to identify commonly regulated ones, data obtained from well-differentiated primary human airway epithelial cultures were considered as reported in Ramachandran et al. [ 25].
Similar results were obtained from sorting comparable differentiated NSC cultures derived from HUES-9 and NDC3.1, as described in Figure 2 and Figure S3 (Fig. 3F,G and data not shown).
In a previous study, primary cell cultures were obtained from two poorly differentiated fibrosarcomas [ 4].
In vivo experiments using the rat sciatic nerve model were performed using the porous hybrid chitosan membranes seeded with neuroglial-like cells obtained from in vitro differentiated N1E-115 cells.
The PROb cell line (DHD-K12-TRb, deposited at the European Collection of Animals Cell Culture, ECACC, Salisbury, UK), obtained from a well-differentiated colon carcinoma induced in the BD-IX rat strain, was cultured in RPMI 1640 medium supplemented with 10% foetal calf serum.
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