Tumours with scores above the obtained cut-off values were considered positive for the expression of the protein.
To evaluate the power of the prediction with F-FDG-PET measured metabolism (parametrically analyzed with SPM, metaROI global index), the authors obtained cut-off scores from an independent sample rather than using cut-off scores present in literature.
Simple logistic regression analysis was performed in order to determine the odds of having pT3-4 or pN1-2 witumorsors below or above the obtained cut-off score from CART analysis.
Receiver operating characteristic (ROC) curve analysis was used to obtain cut-off points.
Hence, to use proteomics-corroborated smORFs to obtain cut-offs would preclude the detection of smORFs translated less strongly, and will negate the main point of doing ribosomal profiling to begin with: to obtain a more extensive, yet sufficiently astringent, repertoire of translated smORFs.
To avoid the subjectivity involved in selecting this or that set of small proteins as benchmarks one could use all smORFs to obtain cut-offs, but this would produce a circular argument (if no smORFs were really translated, their coding sequences would produce very low metrics but still would have a top 90th percentile).
In the clinical field there is a need for obtaining optimal cut offs on markers or tests for separating persons with a specific condition (diseased) from those without this condition (healthy).
Dispersion equations are obtained and cut-off frequencies are determined.
The average filtration velocity has a maximum at an intermediate mass flow rate close to the bed flooding, in contrast to the results obtained by cut-off and time-of-flight methods.
This showed that for WGP in wheat, reliable assemblies cannot be obtained at cut-offs lower than 1e-10.
Due to the size of the data set and the possible number of correlations that can be obtained, the cut-off threshold was set to 0.7.
