Except where noted, BLAST was used to match known and predicted microRNAs to obtain counted expression levels [31] as described in the Methods S1.
We obtained counts of pathogen species from an updated version of the previously published database (1 ).
The authors obtained counts of daily cardiac deaths and of emergency hospital admissions of the elderly for influenza during 1992 2000.
Our obtained counts were subsequently passed through DEGSeq to calculate the differential gene expression (R package version 1.16.0) [ 86].
For each of the 5 years, we obtained counts of the denominator, major (above- and below-knee), and minor amputations.
The obtained counts for each structural feature were then divided by the number of all inserted/deleted amino acids with available structural annotation.
The obtained count per minute values in triplicate was normalized against the positive control of cultures incubated in 10% bovine serum for each experiment.
Counts were further divided by the scan duration to obtain counts per minute per megabecquerel.
Together with the injected dose in our study, SPECT imaging for 30 min may have been insufficient to obtain count statistics for acceptable image quality.
A volume of interest (VOI) was drawn on the reconstructed image in order to obtain counts per second per volume (cc).
