Exact(2)
Following this statistical analysis for identification of de novo mutations, we obtained confident results by excluding false-positive genes.
We obtained confident translations for 6,502 cDNAs containing 20,715 of the 76,568 SNPs.
Similar(58)
The objective of this analysis was to obtain confident parameters to detect crevice corrosion.Noise records obtained from tests where crevice corrosion occurred showed a clear drop in potential along with an increase of current.
It was also difficult to obtain confident results in the measurement of the hydrogen content by reflection electron energy loss spectroscopy; the measurement error was not lower than 20%[11].
We carried out several methods to avoid potential heterogeneity as much as possible and obtain confident results.
A 70% blast hit coverage cut-off (based on subject length) was also applied to obtain confident annotations.
Our Infernal screened yield 709,026 loci matches, and we found that BLAST filtering as well as additional Infernal E-value filtering were needed to obtain confident results.
Although each dog had bilateral lameness, the authors believed that it would only be possible to obtain confident data from the more-lame limbs (lesser PVF), in order to avoid a possible bias caused by inconsistent weight redistribution to the less affected contra-lateral hind limb.
Searches were initially run with a stringent e-value threshold of 1e-35 until convergence (to obtain confident alignments and more robust profiles) and later the threshold was relaxed to the value corresponding to 0.01 times the score of the first false positive and searches were continued until convergence.
Subsampling of real Illumina reads to lower depths indicated ISMapper was able to correctly detect insertions for average genome-wide read depths >20x, although read depths >50x were required to obtain confident calls that were highly-supported by evidence from reads.
Using only annotated genes that contained 10 or more DERs as a cut-off value to obtain confident differentially expressed genes (DEGs) - corresponded to ~14.75-21.61~14.75-21.61%tannotateds - we identified: 3550 DEgenes short thermal stress exposure (STE) vs. control (CTRL); 1875 DEGs for long thermal stress exposure (LTE) vs. CTRL; and 1956 DEGs in Nutrient stress (N) vs. CTRL.
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