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However, as the primer pairs used amplify DBE sequences from many chromosomes (see Additional file 2), the obtained ChIP signals might also derive from genomic localizations of D4Z4 other than 4q.
The obtained chip characteristics in combination with the sensitivity corrected probe-intensity values provide expression estimates scaled in natural units which are given by the binding constants of the particular hybridization.
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In this paper we report an investigation on the processing of magnesium samples by filing and the hydrogen storage properties of the obtained chips.
We obtained ChIP-seq peaks from the C. elegans modENCODE consortium (Araya et al., 2014).
We obtained ChIP-seq data for Stat3 and a GFP control from a previous study (Chen et al, 2008).
To investigate the patterns of DNA methylation on NAT genes and non-NAT genes, we obtained ChIP-seq data sets from GEO.
We obtained ChIP-Seq data of 14 TFs in the K562 cell line and 11 TFs in the GM12878 cell line from the ENCODE consortium [ 3].
In this regard, we have recently obtained ChIP-seq data for sox17 and RNA-seq after sox17 MO knockdown [Cho, Blitz and Zorn, unpublished results].
To evaluate our methods we obtained ChIP-seq data for 11 TFs for which PBM data and tissue specific DNase I hypersensitivity data were available (Additional file 2).
We obtained ChIP-seq datasets for a number of well-established components of the mESC pluripotency network, SOX2, NANOG, KLF4, STAT3, SMAD1, C-MYC, N-MYC, ZFX, E2F1, ESRRB, TCFCP2L1 (Chen et al. 2008), P300 (Schnetz et al. 2010), and BRG1 (Ho et al. 2009), and aligned them to MM8_plus_rDNA.
GeneChip Operating Software (Affymetrix GCOS v.1.4) was used to obtain chip images, with quality control performed using the AffyQCReport software (Bioconductor, Berkeley, CA, USA).
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