In this situation, an x-ray should be obtained, check the lateral x-ray for fragmentation, irregularity, and an ossicle of the tibial tubercle.
The column was eluted with chloroform and the fractions obtained were checked for their purity by performing thin layer chromatography.
Two Δgox transformants were obtained and checked for the deletion of the putative gox gene by 3 different pairs of primers (Table 1).
cDNA obtained was checked for the mRNA levels of cryptidin1, 4 and 5 with mouse β-actin mRNA levels as internal control.
Transformed colonies obtained were checked for protein expression with the help of IPTG (1 mM).
Transformed colonies obtained were checked for protein induction by IPTG (1 mM).
The Illumina reads obtained were checked for overrepresented sequences or artifacts, but none were found.
Boxplots, scatterplots and histogram outputs of the normalised data were obtained and checked for consistency (not shown).
After sporulation and clonal selection, homokaryotic G418 r transformants were obtained and checked for their leucine auxotrophy.
Studies were reviewed initially on the basis of title and abstracts, and then all full manuscripts for those that appeared relevant were obtained and checked for eligibility.
