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The obtained bands at 1,661 and 1,531 cm−1 for the amide I and amide II, respectively, also confirm the alpha helical structure of casein protein (Luginbuhl 2002).
To identify the lysine residue involved in the mono-ubiquitination, we co-transfected cells with the mutant USP25mC178S with His(6x Ub, enriched the lysate in USP25m forms by immunoprecipitation with an anti-cMyc antibody, and analysed the obtained bands by LC-ESI-QTOF mass spectrometry.
For testing the specificity of the obtained bands, inhibition tests were performed with the appropriate antigens.
The intensities of the obtained bands were determined using Printgraph AE-6920-MF AE-6920-MF AE-6920-MF
Densitometry analysis of the obtained bands was performed using the SynGene software.
We successfully amplified the COI in the samples and obtained bands of approximately 800 bp on the gel.
Similar(51)
The obtained band gap of 2.46 eV agrees well with the experimental value of 2.51 eV.
The obtained band dispersion well agrees with the previous study [31].
Thus, the obtained band gap agrees well with the reported bulk value (3.37 eV) at room temperature.
The obtained band dispersion well agrees with the previous studies, while the existence of the quasi-flat band was not referred [19, 28].
The obtained band gap energy is an accepted value for the photocatalytic activities in visible light and also for application in the photovoltaic and optical devices.
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