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However, a certain extent of the amplification results from the compression effect induced by the counter flow, and the obtained amplification level is difficult to be measured in the experiments.
Based on the obtained amplification curves, the Ct values (fractional cycle number at threshold), the value of PCR efficiency and the corresponding coefficient of determination (R2) were calculated using LinRegPCR software (Ruijter et al., 2009) for all four samples (IR64C, IR64S, IR64-Sub1C and IR64-Sub1S).
Cloning and sequence analysis of all obtained amplification products confirmed the absence of full-length asp f16, but identified a novel variant, which we named crf2 due to its partial similarity to the published crf1 [31].
We obtained amplification products of 300 400 bp, in agreement with the expected sizes.
We obtained amplification until a minimum concentration of 3 ng of viral particles.
The obtained amplification curves from unpurified DNA samples with all possible outcomes for each probe are shown in Figure 1.
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The conventional parameter Vs30; averaged shear wave velocity in the top 30 m used in current design codes, did not correlate well with the obtained amplifications.
We were unable to obtain amplification products from 1 nasopharyngeal sample.
All sequences and mixed positions are shown in Table 2. Typically it is very difficult to obtain amplification products longer than 100 bp from ancient remains and bone material that have been buried for a long time.
Specific chtB primers were used to obtain amplification.
Cycling conditions were optimized to obtain amplification within the exponential phase.
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