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Worms obtained alive were fixed approximately 1 to 3 days after their removal from the host, while worms fixed prior to shipping were typically used 1 to 2 weeks after reception.
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Nor can a dead man hope to gain from anybody that which he did not obtain while alive in the world.
196/03 (Codex on Privacy) a written consent was obtained from the alive patients.
The only follow-up information required was the date of death or the date of last contact/last known to be alive, obtained from the tumor registries at MRMC and SRMC.
Blood samples were collected from the tail vein of each rat under short isofluraneanesthesia before treatment and after 1, 2, 3, 6, 9, 12, 15, 18, 21, and 24 months:11 measurements were obtained for each animal alive at 2 years.
Following an initial period for populations to establish, time series for all species were obtained by counting both alive and dead insects each week from every patch (dead insects were then removed).
Signed informed consent for translational research was obtained from patients still alive.
Vital status (dead or alive) was obtained from the Civil Registration System.
Fully informed consent was obtained from all patients alive at the time of outcome data collection.
The use of patient materials was approved by the Ethical Committee (Ethical permit; Dnr 00-343) athehe Karolinska Institutet and informed consent was obtained from all patients alive at the time of analysis.
P. balsamifera was chosen for this study because of the local availability of trees for this and future studies, the very close relationship of Populus balsamifera and P. trichocarpa [ 2], the large amount of genetic variation among P. trichocarpa trees [ 1, 3], and the fact that the P. trichocarpa tree for which the genome sequence was obtained is no longer alive.
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