The study could be carried out only in smaller number of subjects due to the difficulty in obtaining adipose tissue samples.
Blood glucose levels were determined at 0, 2, and 7 days, and diabetic mice were sacrificed to obtain adipose tissue, the pancreas, kidney, liver, spleen, skeletal muscle, and intestines.
In order to obtain adipose tissue venous blood, a 10 cm 22-gauge catheter (Central venous catheter kit Seldinger technique; Becton Dickinson, Alphen aan den Rijn, the Netherlands) was introduced anterogradely over a guide wire into one of the superficial veins and threaded towards the groin, so that its tip lay just superior to the inguinal ligament [ 15].
To obtain adipose-derived MSCs, raw human adipose tissue collection and cell harvests were approved by the Chinese People's Liberation Army General Hospital's Protection of Human Subjects Committee.
Stromal vascular fraction (SVF) is an autologous source of heterogeneous cell population obtained from adipose tissue, which is rich in stem cells and presents little immunogenicity to the host.
The study recruited and obtained visceral adipose samples from 20 surgical patients [7 lean (BMI<30), 7 obese non-treated (OB; BMI≥30), and 6 obese treated (OBTD) patients].
A fat sample obtained by adipose tissue biopsy revealed 3,200 mg/kg Arochlor 1254.
In addition, the cell number obtained from adipose tissue is usually adequate for transplantation [ 10].
Similar results have also been obtained for adipose tissue (Belanger et al., 2002).
Resonances corresponding to the carbon atoms of triacylglycerides were obtained when adipose tissue constituted more than 10% of the carcinoma.
