They also provided this interesting tidbit: A person need only ingest as little as three teaspoons of the water before becoming "almost certain" to obtain a virus of some sort, whether it be of the stomach, respiratory or even heart or brain variety.
In contrast, the WT702 was readily detected in the lung homogenates of quail infected under similar conditions, and the lung homogenates were used to obtain a virus better adapted to quail.
We plated serial dilutions from the microtiter plate on a lawn of Pp to obtain a virus concentration for each sampling point.
Such a study would increase the likelihood of obtaining a virus isolate and would minimize the potential adverse impact on the bat populations from oversampling large numbers of otherwise healthy bats.
To this end, 125 μl of the test mixture was added to the MicroSpinTM column and centrifuged at 737 g for 1 min. Subsequently, 120 μl of the eluted suspension was mixed with ice-cold cell culture medium to obtain a virus dilution of 10-1.7 10-1.7 prepand serial ton-fold dilutions uprepare-7.7.
Dzianott and Bujarski [ 37] obtained a Brome mosaic virus growth curve in A. thaliana.
As a consequence, the mutant virus obtained a lower fitness level than the wildtype virus.
Dr. Eckard Wimmer, professor of molecular genetics and microbiology at Stony Brook and leader of the project, said they made the virus to send a warning that terrorists might be able to make biological weapons without obtaining a natural virus.
In most cases, he said, it would be easier to obtain a natural virus than to build one from scratch.
