In practice, to obtain a validation dataset of reasonable size, we selected bulls born in the three most recent years in the pedigree (2003, 2004 and 2005).
In order to obtain a validation set of CNVs, we applied GMM and the PCA-based merging algorithm to these data.
In addition, we used qRT-PCR on some of the stable transformants expressing STOX1 at different levels, and obtained an external validation of the microarray results (Figure 2).
We obtained an experimental validation rate 85% (137 out of 161) for SNPs detected in our white spruce contigs at PSNP ≥ 0.95.
Barbazuk et al., [ 13] obtained an 85% validation rate when using a 454 GS-20 run to sequence the transcriptome of two inbred maize lines when there was no reference genome sequence available.
In addition to the WTCCC dataset, we obtained an independent validation dataset from the Type 1 Diabetes Genetics Consortium with 4844 cases and 3552 controls (without any overlap with the 5004 samples of the WTCCC dataset).
In sum, among ESRP1-dependent exon skipping events identified by MADS+, we obtained a RT-PCR validation rate of 88.5% (153 exons validated out of 173 tested so far, Warzecha,C.C. et al., unpublished data).
Several typical organics including ethanol, 1-butanol, p-cymene and liquid paraffin are used as precursors to react with ammonium persulfate (APS) in an autoclave, and carbon particles are obtained as a validation.
The ability of the MTSDs to estimate the mean AWS1m in a soil profile was generally acceptable for each sub-profile, as indicated by the RMSD and RBIAS values obtained from a validation dataset, which ranged from 3.6 to 7.7 mm and from 0.07 to 0.13, respectively, among the four land use types.
