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Meanwhile Franklin proceeded with Lieutenant Fowler to Canton, where he obtained a passage to England in the Earl Camden, East Indiaman, commanded by Sir Nathaniel Dance, commodore of the China fleet of 16 sail.
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Therefore, we obtained an early passage of the original 184 primary cell pool transduced with hTERT [ 17].
At the ninth passage, we obtained a stable population of these cells, thereafter named "Speedy".
Through the help of some Dinka tribesman he was able to acquire a Sudanese passport on the black market and obtain a ticket for passage to Cairo.
Next, we examined the properties of BSE PrPSc obtained from passage of an H-type isolate of BSE from a cow with a polymorphism at codon 211 of PRNP (U.S. 2006 BSE case) to a calf also heterozygous for the E211K polymorphism [ 19]. (As this PrPSc may contain a mixture of E211 and K211 protein, we refer to this isolate as E211K and cattle carrying the mutation as EK211).
Single cell suspensions of CB6F1 spleens were obtained by passage through a fine metal mesh filter.
Single splenocyte suspensions of CB6F1 mice were obtained by passage through a fine 100 µm nylon cell strainer (BD Pharmingen, USA).
By using the same tactics of aggressive leadership, Roosevelt in 1906 also obtained passage of a Meat Inspection Act and a Pure Food and Drug Act.
He procured legislation that set up an arbitration board to settle work disputes and obtained passage of a law that fined employers who fired workers for belonging to a union.
Cells were serially passaged to obtain a sufficient number of cells and used between the first and second passages.
Bone marrow-derived hMSCs were obtained as passage 1 in a cryovial from the Center for the Preparation and Distribution of Adult Stem Cells.
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