The pLAC11 vector can be used to conduct physiologically relevant studies in which the cloned gene is expressed at levels comparable to that obtainable from the chromosomal copy of the gene in question.
Using an increased number of replicates for the low concentration samples firstly allowed us to have a reliable reference for the Cq value obtainable from one single copy of the mutant allele and secondly, produced an increased amount of data which facilitated the use of a statistics-based approach for the estimation of the average mutant JAK2 copy number (see below).
Running 10 replicates of the 0.01% MUT/WT mix in each PCR run allowed us to have a reliable reference for the Cq value obtainable from one single copy of the mutant allele, which was then used as a guideline for the identification of rare false-positive occurrences.
The identical copies just needed to be obtainable.
A separate edition of this document was produced by a method that assures numerous identical and durable copies, and those copies were simultaneously obtainable (from the publication date listed on page 1 of this article) for the purpose of providing a public and permanent scientific record, in accordance with Article 8.1 of the Code.
A separate edition of this document was produced by a method that assures numerous identical and durable copies, and those copies were simultaneously obtainable (from the publication date listed on page 1 of this paper) for the purpose of providing a public and permanent scientific record, in accordance with Article 8.1 of the Code.
Therefore, a separate edition of this document was produced by a method that ensures numerous identical and durable copies, and those copies were simultaneously obtainable (from the publication date noted on the first page of this article) for the purpose of providing a public and permanent scientific record, in accordance with Article 8.1 of the Code.
Therefore, a separate edition of this document was produced by a method that assures numerous identical and durable copies, and those copies were simultaneously obtainable (from the publication date noted on the first page of this article) for the purpose of providing a public and permanent scientific record, in accordance with Article 8.1 of the Code.
In addition, copies of editorial rejection letters were obtainable.
The integral copy numbers for the classified sequences are the by-product of our model's classification task, obtainable by an efficient Viterbi-like max-product algorithm.
