Exact(5)
Cftr mice were bred to obtain wild type (WT) [Cftr] and CF [Cftr] mice.
N. furzeri was selected because the distribution range of this species overlaps with a cline in altitude and precipitation/evaporation. We are unable to provide a definitive conclusion, as we could not obtain wild fish from GRZ (Zimbabwe), i.e., the northern end of this cline.
CFTR mice were bred to obtain wild type [CFTR] and CF [CFTR] mice.
This is because it was not possible to obtain wild sexually mature normal fish from the studied populations.
C57BL/6 IL-23p19-deficient IL-23p19-deficient IL-23p19-deficientn Francisco, CA, USA) and backcrossed to BALB/c mice obtained from National Cancer Institute (Bethesda, MD, USA) for eight generations, and were then intercrobtained obtain wild type (WT) and p19−/− littermates.
Similar(55)
Over the years "new blood" was added by introducing bats from other Zoos and further expeditions to obtain wild-caught animals to increase the genetic diversity of the colony.
The mice are in C56BL/6;129svj background and were intercrossed to obtain wild-type (BAMBI+/+) and BAMBI-deleted (BAMBI−/−) mice.
The heterozygous mice (F1 mice) were interbred to obtain wild-type, heterozygous, and homozygous littermates (F2).
To obtain wild-type FLAG-WNK4, we utilised the cell lines from Schumacher et al (2014) and Ohta et al (2013).
Gdap1flox/flox mice were crossed with cre-deleter lines to ablate GDAP1 expression in the germline and bred to obtain wild-type, heterozygous and knockout animals (Gdap1 wt/wt, Gdap1 wt/- and Gdap1−/−).
The resultant chimeric mice were used for the foundation of a transgenic line and a breeding strategy was used to obtain wild-type mice and mice that were either heterozygous or homozygous for the mutation.
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